![]() ![]() ARG62346 anti-beta Actin antibody BA3R WB image Western blot: Rat brain, Rat heart, Rat kidney, Rat stomach and Rat ovary lysates stained with ARG62346 anti-beta Actin antibody BA3R at 1:1000 dilution. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.Western Blot Positive Control Actin, cytoplasmic 1 AntibodyĪctins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Western blot: MCF-7, A549, H1299, HCT116, HepG2 and HUVEC cell lysates stained with ARG62346 anti-beta Actin antibody BA3R at 1:1000 dilution. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. Actins are highly conserved proteins that are involved in various. Reactivity to Drosophila was only verified with the purified format. Purity, Antigen affinity UniProt, Q7ZVI7 Applications, Western blot : 1:1000 Description. It is recommended that the reagent be titrated for optimal performance for each application. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg/ml in human, mouse, rat and zebrafish and 0.1 - 0.5 µg/ml in Drosophila. The antibody solution should be stored undiluted between 2☌ and 8☌.Įach lot of this antibody is quality control tested by Western blotting. The antibody was purified by affinity chromatography. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Enhanced chemiluminescence was used as the detection system. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4☌, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. Lane 1: Molecular weight marker Lane 2: 20 µg of Drosophila head lysate Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. Western blot of purified anti-β-actin antibody (clone W16197A). Actin is a 43-kDa protein that is found in nearly all eukaryotic cells at concentrations greater than 100. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. Total lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:800 diluted (0.25 µg/mL), 1:1600 diluted (0.125 µg/mL), 1:3200 diluted (0.0625 µg/mL), 1:6400 diluted (0.03125 µg/mL) and 1:12800 diluted (0.0155 µg/mL) purified anti-β-actin antibody (upper). Because -actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). ![]() ![]() Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain and zebra fish were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 diluted (0.1 µg/mL) purified anti-β-actin antibody (upper). ![]()
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